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five–four μmol/L vary,[156][157] the focus of serotonin in synaptic vesicles was measured at 270 mM.[182] pursuing vesicular release, the ensuing concentration of serotonin while in the synaptic cleft, to which serotonin receptors are uncovered, is approximated to be about 300 μmol/L. Consequently, while in vitro receptor binding affinities,